Sirna Library Screening

To better understand this complexity researchers are moving toward large screening projects to identify candidate genes involved in their biological question of interest.

Sirna library screening.

Skip to primary content. A breast cancer cell line was transfected with a sirna library that targeted a variety of specific kinases. Sirna libraries composed of large numbers of sirnas have been developed and are used to identify sets of genes functionally involved in a particular biological process or disease. 1 transfection of cells with sirna pools is performed using reverse transfection.

The number of patients with cell types selleck screening library determined according to world health organization criteria were 2 5 7 37 and 7 for types a ab b1 b2. Take advantage of predefined gene families including druggable genes or whole genome. Cancer research 2012 14 r22. Learn more and view all available silencer sirna libraries.

Sirna library minimize time cost of screening using highly functional sirna. Fast and easy online configuration and ordering of plated sirna microrna reagents. Freedom with custom library design. Rna interference rnai is a conserved biological response to the presence of double stranded rna which mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids and regulates the expression of protein coding genes.

Genome wide sirna and microrna libraries. Sirna library screening protocol. Adapted from hu et al. Screening often lies at the beginning of most discovery research.

Sirna library provides a new and efficient way that bring about many original breakthroughs in biology especially for the discovery of new targets combined with. The network of possible molecular interactions leading to the causal phenotype within a given system pathway or disease is astounding. Rnai screening has allowed researchers to study the functionality of genes in a cell both individually and in. Prepare sirna liposome complexes in the wells of 96 well plates.

The silencer select human apoptosis sirna library provides investigators with a focused sirna screening platform targeting the most up to date apoptosis gene targets thus enabling a detailed analysis of the apoptotic signaling pathway the silencer select human apoptosis sirna library contains three unique non overlapping sirnas per target for 924 genes. It enables high throughput low cost ultra fast gene function screening disease mechanism research target discovery drug development etc. Silencer sirna is a great choice for economical screening of pre defined gene families and popular pathways like kinases cell surface receptors and dna damage response.